Glucose is usually used by cells for energy.
Around the sides of the tank are symbols, and the goal entails (pun intended) that the rats remember the location of the platform, to save themselves from exerting energy to stay afloat by refinding the platform and emerging from the water. In the Morris test, rats are trained to find a submerged platform in a tank of water.

In contrast, diabetic rats treated chronically with orally administered berberine at doses of 25, 50, or 100 mg/kg, but especially the 50 and 100 mg dose, twice daily for 30 days, and then subjected to the Morris water maze test, were all found to have improved cognitive performance, along with lowered hyperglycemia, oxidative stress, and ChE activity.

The researchers used the Morris water maze model as a measure of memory. In the second leg of the Indian study, 30 days after confirmation of diabetes, berberine was given at the same dose range given during training trials in the Morris water maze that lasted 5 days (days 31-35).
Alternatively, vitamin C at 100 mg/kg or metformin at 500 mg/kg, or donepezil (an AChEI that is similar to the plant nutrient galantamine) at 3 mg/kg or placebo at 1 ml/kg were also used.

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